(345) Optimized HPLC Method for TX & FV Estimation in Plasma & Brain: Pharmacokinetics & Biodistribution

Introduction: Glioblastoma multiforme (GBM) is an aggressive brain tumor with limited treatment options. This study explores a novel therapeutic strategy by combining the anti-mitotic drug TX and natural compound FV, known for their synergistic anti-cancer effects. Both drugs were loaded into nanostructured lipid carriers (NLC) for enhanced delivery. A validated HPLC method was developed for precise estimation of TX and FV in plasma and brain. Pharmacokinetic and biodistribution study showed improved bioavailability and distribution via intranasal delivery, highlighting potential for targeted GBM treatment.