(123) Endoplasmic reticulum stress-based approach for reprogramming tumor-derived exosome

Introduction: Despite their potential of Tumor Associated Antigens (TAA)-based cancer vaccine, most TAAs require additional adjuvants. Tumor-derived exosomes (TEX) possess unique characteristics, containing TAA and DAMPs that can function as adjuvants. However, TEX presents a double-edge due to their inherent immunosuppressive proteins (ISP). So, we developed a novel approach by pre-conditioning cancer cells using endoplasmic reticulum stressor for the purpose of generating immunogenic exosomes (iTEX). The designed iTEX can effectively deliver TAAs and DAMPs to dendritic cells while mitigating ISP.