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Bioengineering (Focus Group – BE)

(127) Interactions of biomimetic liposomes with enterocytes explored by 3D Raman imaging

Introduction: Outer membrane vesicles (OMVs) are a diverse group of phospholipid nanovesicles actively secreted by bacteria. Certain OMVs have demonstrated the ability to withstand the gastrointestinal tract environment and traverse biological barriers. This capability positions OMVs as promising carriers for oral drug delivery. Here, we introduce the bottom-up assembly of artificial biomimetic liposomes (BLs), mimicking the lipid composition of naturally occurring OMVs. Using 3D Raman imaging, we comparatively evaluated the interaction of naturally occurring OMVs and BLs with the intestinal epithelium.

Learning Objectives:

  • produce synthetic BLs mimicking the complex lipid composition of OMVs in a bottom-up approach.
  • conduct semi-quantitative analysis of time-dependent vesicle uptake into cells by 3D Raman imaging.
  • demonstrate that BLs exhibit higher stability in the cellular environment and increased uptake.