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Gene Delivery and Gene Editing (Focus Group - GDGE)

(171) Tracking Endosomal Escape of Tunable Polymer Nanoparticles for Inhibition of Inflammatory Pathways

Introduction: Endothelial cells (ECs) are an important target for immunomodulation, acting as governors in the inflammatory response [1]. Polymer nanoparticles (NPs), can be used to deliver nucleic acids into cells to modulate their immune activity [2]. Endosomal escape (EE) of NPs is critical to the success of treatment [3]. The goal of this study was to develop a high throughput method to track NPs through the endocytic pathway in ECs. These methods aimed to discover characteristics of NPs that lead to the greatest EE and subsequent therapeutic activity.

Learning Objectives:

  • Understand how timing of the endocytic pathway affects gene knockdown and immunomodulation in ECs.
  • Evaluate how NPs chemical differences influence cellular uptake, EE, and therapeutic activity.
  • Discern how knockdown of an inflammatory gene in ECs can affect pathways and downstream molecules.