(175) Microfluidic Erythrocyte-Camouflaged Chitosan Nanoparticles for Nucleic Acid Delivery
Introduction: Gene therapy holds immense potential for treating a wide range of diseases but delivering nucleic acids safely and effectively remains a major challenge [1]. These molecules are prone to rapid degradation and nanoparticles (NP) designed for their delivery are often cleared by the immune system [2]. In this study, chitosan-based DNA NP coated with red blood cell membranes (RBCm) were formulated and prepared by microfluidic technique, through a scalable solution that enhances stability, prolongs circulation time and paves the way for more effective gene therapy applications.
Learning Objectives:
Red blood cell membranes coated chitosan nanoparticles for long circulation gene carriers.
Angela Fabiano – Associate Professor, Pharmacy, University of Pisa; Ylenia Zambito – Full Professor, Pharmacy, University of Pisa; Siria Emily Nesti – PhD student, University of Pisa; Rebecca Piccarducci – Researcher, Pharmacy, University of Pisa; Laura Marchetti – Associate Professor, Pharmacy, University of Pisa; Lorenzo Arrico – Researcher, Chemistry, University of Pisa; Vanessa Susini – Technician, Translational Research and New Technologies in Medicine, University of Pisa; Chiara Giacomelli – Associate Professor, Pharmacy, University of Pisa; Lorenzo Di Bari – Full Professor, Chemistry, University of Pisa; Maria Franzini – Assistant Professor, Pharmacy, University of Pisa; Anna Maria Piras – Assistant Professor, Pharmacy, University of Pisa
