(180) Real-Time Label-free study of spherical nucleic acids for delivery of antisense TAUoligonucleoutides
Introduction: Spherical nucleic acids (SNAs) are promising nanocarriers for gene therapy due to their unique properties. They consist of a three-dimensional structure with an inorganic core, as Buckminsterfullerene, and oligonucleotides (ONs) on the surface. SNAs can be functionalized to improve properties like membrane permeability. In this project, SNAs are multifunctionalized with DNA-based ONs and hybridized with TAU antisense oligonucleotides (AONs). Surface Plasmon Resonance technology is used for in vitro assessment, enabling label-free, real-time analysis of SNA properties and cell interactions.
Learning Objectives:
The project aims to use one detection technique to analyze nanoparticle-based delivery systems.
To minimize the need for multiple techniques, reducing development costs for these nanosystems.
To focus on the properties and interactions of promising nanoparticle-based drug/gene systems.
