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Gene Delivery and Gene Editing (Focus Group - GDGE)

(187) Layered F-PEI Nanoparticles for Targeted Delivery of TXNDC5 Silencing for Pulmonary Fibrosis

Introduction:

Pulmonary fibrosis (PF) is a progressive lung disease characterized by excessive extracellular matrix deposition, leading to respiratory failure. TXNDC5, a disulfide isomerase upregulated in PF, contributes to fibroblast activation and collagen production. This study explores a fluorinated polyethyleneimine (F-PEI)-based nanoparticle (NP) system layered with hyaluronic acid (HA), polyethylene glycol (PEG), and a PDGFRB-targeting peptide to deliver shRNA against TXNDC5. This targeted delivery system aims to suppress fibrotic gene expression and mitigate PF progression.

Learning Objectives:

  • Explain how F-PEI complexes and protects TXNDC5 shRNA for targeted gene silencing in IPF.
  • Describe how the HA-PEG-PDGFRB shell boosts NP stability and targets fibrotic cells.
  • Evaluate TXNDC5 knockdown effects on reducing fibrotic markers in pulmonary models.