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Gene Delivery and Gene Editing (Focus Group - GDGE)

(199) Systematic Optimization of Cationic Liposomes for Stable and Efficient mRNA Delivery

Introduction:

Messenger RNA (mRNA) therapeutics show great promise for gene therapy and vaccination. However, they face substantial delivery challenges, including instability, rapid degradation and limited cellular uptake (1). Cationic liposomes have emerged as a valuable non-viral vector, forming electrostatic complexes that protect and deliver mRNA intracellularly. However, issues such as aggregation, low encapsulation efficiency and suboptimal transfection remain problematic (2). We aim to optimize liposomal formulations to balance stability and efficiency, thereby advancing mRNA delivery strategies.

Learning Objectives:

  • Recognize key factors impacting mRNA stability and delivery in cationic liposomes.
  • Understand how composition influences liposome stability, encapsulation efficiency and transfection.
  • Compare optimized cationic liposomes to commercial reagents for mRNA-based applications.