(202) Design of PD-L1-Targeted Lipid Nanoparticles to Turn on PTEN for Efficient Cancer Immunotherapy
Introduction: Lipid nanoparticles (LNPs) exhibit remarkable mRNA delivery efficiency, yet their majority accumulate in the liver or spleen after injection. Tissue-specific mRNA delivery can be achieved through modulating LNP properties, such as tuning PEGylation or varying lipid components systematically. In this paper, we used a streamlined method for incorporating tumor-targeting peptides into the LNPs; the programmed death ligand 1 (PD-L1) binding peptides were conjugated to PEGylated lipids via a copper-free click reaction, and directly incorporated into the LNP composition (Pep LNPs).
Learning Objectives:
Understand the role of PD-L1-targeted LNPs in enhancing tumor-specific mRNA delivery.
Evaluate the impact of PTEN/Pep LNPs on anti tumor immune activation and therapeutic efficacy.
Analyze the advantage of copper-free click chemistry for peptide conjugation in targeted LNP design.
