(207) MMP9 gene silencing by siRNA delivery using hybrid nanoparticles reduced the migration of TNBC cells

Introduction: Triple negative breast cancer (TNBC) is the most aggressive subtype and breast cancer metastasis is the main cause of mortality (1, 2). Enzymes such as matrix metalloproteinases (MMP) that possess proteolytic activity increase cell mobility due to extracellular matrix degradation, which plays a crucial role in metastasis. Authors associated MMP overexpression to a malignant phenotype (3, 4), and previous report showed overexpression of MMP9 in TNBC (5). Therefore, we used hybrid nanoparticles (NP) to deliver MMP9 siRNA, to knockdown MMP9 gene and to evaluate the RNAi effects in TNBC cells.