Gene Delivery and Gene Editing (Focus Group - GDGE)

(214) Novel Ether-Ionizable Lipids Complexed into Lipid Nanoparticles Enhance mRNA Delivery Efficiency

Introduction:

The ionizable lipid within lipid nanoparticles (LNPs) is pH-responsive, enabling cytosolic nucleic acid delivery [1,2]. Ionizable lipid design drives nucleic acid delivery efficiency [3], but structure:function relationships between lipid design and LNP delivery are not well understood. We synthesized a series of novel ionizable lipids with different numbers of ether groups and alkyl-tail lengths and identified top-performing ionizable lipids for mRNA delivery to cells. This work aims to understand how ether groups and tail length influence delivery while providing new lipids for diseases.

Learning Objectives:

Understand relationships between number of ethers and tail lengths and mRNA delivery.
Assess how ionizable lipid structure influences endocytic pathways.
Recognize that ether groups can greatly alter biodistribution in mice.

Poster Presenter(s)

Joshua Yang, n/a

Doctoral Student
Rowan University
Cherry Hill, New Jersey, United States