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Immuno Delivery (Focus Group - ID)

(246) Design of experiments-based development and in vitro evaluation of a triple adjuvanted nanovaccine

Introduction:

Background and Purpose:  A lipid nanoparticle-triple adjuvant complex (L-TriAdj) is being explored for intranasal pertussis vaccines to enhance mucosal immunity.[1,2] The lipid composition is critical to modulate cytotoxicity, uptake and cytokine expression from antigen presenting cells. The NPs were comprised of DDAB/DOPE/phospholipid/cholesterol, with NP size optimized by design-of-experiments methodology. The hypothesis was that phospholipid alkyl chain length (DPPC vs. DSPC), would be related to cellular uptake and stimulation of dendritic cell expression of immune stimulating cytokines.

Learning Objectives:

  • explain how DOE can identify lipid NP compositions with desirable particle size and zeta potential
  • relate the lipid composition of vaccine lipid nanoparticles to their physicochemical properties
  • relate the lipid composition of vaccine NPs to the immune activation of antigen presenting cells