(252) Enlighting the black box: Nanocarrier formation inside microfluidic mixing devices

Introduction: The successful market authorization of nanocarrier formulations remains rare, primarily due to the lack of scalable and reproducible fabrication methods. Microfluidic-based manufacturing has emerged as a promising approach, particularly for lipid-based nanoparticles encapsulating RNA payloads. These processes typically rely on the controlled dilution of ethanolic lipid solutions with aqueous buffers. However, the dynamics of this dilution and the actual nanocarrier formation within microfluidic channels are poorly understood, critically limiting their optimization and reproducibility.