(266) Effect of freezing conditions, cryoprotectant concentrations, and PVP on crystallization
Introduction: Understanding the effects of freezing conditions, cryoprotectant concentrations, and the presence of polyvinylpyrrolidone (PVP) on crystallization behavior is essential for optimizing mRNA and protein-based therapeutics. Freezing conditions impact crystallization by affecting nucleation and growth kinetics. Fast freezing leads to smaller, less-ordered crystals due to the limited time for molecular arrangement, whereas slow freezing allows for more organized crystal formation. The cooling rate also influences the degree of supercooling, which affects the polymorphic forms of the crystals [1].
Learning Objectives:
evaluate how freezing protocols affect crystallization.
assess the role of PVP in controlling crystallization.
analyze XRD data to understand crystallization behavior.
Xiufang Cheng – Graduate Research Student, Department of Pharmaceutical Sciences, Saint Joseph's University; Jasmin Monpara – Senior Research Fellow, Department of Pharmaceutical Sciences, Saint Joseph's University; Sriramakamal Jonnalagadda – Professor of Pharmaceutics, Department of Pharmaceutical Sciences, Saint Joseph's University; Pardeep Gupta – Professor of Pharmaceutics, Department of Pharmaceutical Sciences, Saint Joseph's University; Rijo John – Senior Research Fellow, Department of Pharmaceutical Sciences, Saint Joseph's University
