(296) Enhancing mRNA-LNP Efficacy: Investigating the Role of Ionisable and Cationic Lipid Combinations
Introduction: Lipid nanoparticles (LNPs) normally consist of neutral phospholipids, cholesterol, ionizable lipids, PEG-lipids, and nucleic acids, each contributing to LNP stability, transfection efficiency, and safety (1). Ionizable lipids, like SM102, have an optimized pKa value that ensures they are neutral at pH 7.0 but become cationic at lower pH (2). This study aims to further explore strategies for improving LNP potency by combining ionizable lipids with fixed cationic lipids in LNP formulations.
Learning Objectives:
Demonstrate the preparation and physicochemical characterization of DDAB LNP formulations.
Evaluate the in vitro expression and cell viability of mRNA LNPs in HEK293 cells.
