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Nanomedicine and Nanoscale Delivery (Focus Group - NND)

(298) Behavior of Lipid Nanoparticles in Aqueous Formulations

Introduction: The rise of lipid nanoparticle (LNP) formulations calls for tools to predict their colloidal stability with excipients. The diffusion interaction parameter (kD) from dynamic light scattering (DLS) measures particle attraction/repulsion, aiding stability insights.While applied to proteins, it’s unexplored for LNPs.kD is calculated from diffusion coefficients at varying concentrations. The apparent hydrodynamic radius at infinite dilution (RH,0) reflects excipient effects on LNPs physical state. This study compares the diffusion coefficient, kD and RH,0 in lysozyme and LNPs with sodium chloride.

Learning Objectives:

  • LNPs do not behave like charged or particles with mixed positive and negative charges in solution.
  • Salt concentration effects the RH,0 of LNPs.
  • Salt concentration does not affect the kD of LNPs.