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Nanomedicine and Nanoscale Delivery (Focus Group - NND)

(364) Evaluation of triazine-based lipid nanoparticle (LNP) formulation for mRNA delivery in a mouse model

Introduction:

Lipid nanoparticles (LNPs) are the most prominent platform for mRNA delivery.1 The primary component of an LNP is the ionizable lipid that enables mRNA encapsulation, and transfection efficiency. Novel lipids with tailorable physicochemical properties provides an opportunity to explore structure activity relationships of the lipids. Herein we evaluated the performance of novel triazine (TZ)-based ionizable lipids,2 for mRNA transfection efficiency in vitro and in vivo. Our results demonstrate TZ lipids as an efficient platform to evaluate screen for improved mRNA delivery.

Learning Objectives:

  • Evaluate the physicochemical properties of triazine-based ionizable lipids in mRNA-LNP formulations.
  • Assess mRNA transfection efficiency and cell viability of the mRNA-LNP formulation in vitro.
  • Examine mRNA expression in mouse after intramuscular and systemic injection of mRNA-LNP formulation.

    Poster Presenter(s)

  • Nabilah Ibnat, n/a

    Postdoctoral Scholar
    University of Kentucky
    Lexington, Kentucky, United States