Skip to main content
CRS 2025 Annual Meeting & Exposition Main banner
Rate Poster

Nanomedicine and Nanoscale Delivery (Focus Group - NND)

(368) Refining peptide-functionalized LNPs for enhanced tumor-targeted mRNA delivery

Introduction:

We designed peptide-functionalized lipid nanoparticles (LNPs) for tumor-targeted mRNA delivery upon intravenous application. A key challenge lies in accurate quantification of ligand density and comparability of targeting efficacy and transfection. We established a platform-based approach, to improve characterization and enable cost-effective mRNA generation, allowing for informed particle design for active targeting to tumors. RGD peptides - which are prototypic ligands for the αvß3 integrin receptor that is overexpressed on various tumor and endothelial cells - are used as model peptides.1

Learning Objectives:

  • Assess the suitability of peptide quantification assays for functionalized LNP formulations
  • Describe a cost-efficient approach for custom mRNA generation for in vitro and in vivo applications