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Nanomedicine and Nanoscale Delivery (Focus Group - NND)

(392) In Vitro Evaluation of Ionizable Lipids in Lipid Nanoparticle (LNP) Formulations.

Introduction:



Introduction: Lipid nanoparticles (LNPs) are essential for delivering nucleic acids, especially mRNA vaccines, by safeguarding mRNA from degradation, improving cellular uptake, and facilitating efficient protein translation (1,2). Composed of ionizable lipids, structural lipids such as DSPC, cholesterol, and PEG-lipid conjugates, LNPs are stabilized for enhanced biodistribution (3). This work aims to investigate how different ionizable lipids affect LNP performance in vitro.

Learning Objectives:

  • Upon completion, participants will be able to compare particle sizes of different lipid formulations
  • Upon completion, participants will be able to evaluate encapsulation efficiency and mass balance
  • Upon completion, participants will be able to analyze cellular expression across different cell line