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Nanomedicine and Nanoscale Delivery (Focus Group - NND)

(395) Quantification of Payloads in Lipid Nanoparticles by Scatter-Free UV/Visible Absorption Spectroscopy

Introduction:

UV/Vis spectroscopy is used for RNA, DNA, and protein quantification at 260-280nm. In Lipid Nanoparticles, particle scattering affects the spectrum, complicating UV quantification or requiring complicated particle disruption steps [1]. To address this bottleneck in nanomedicine development we introduce Scatter-Free Absorption (SFA) spectroscopy for accurate LNP payload without disruption or dyes. This new method requires almost no sample prep, only 2µg of material and achieves ca. 2% precision. Compared to RiboGreen, SFA offers results in seconds, ease of use, high throughput, and reliability.

Learning Objectives:

  • Discover a new reliable alternative method to fluorescence assays for LNP payload quantification.
  • Learn how LNP quantification methods compare for speed, ease of use, accuracy and precision
  • Understand how much this technology could speed up development and manufacturing workflows