Tech Session III: Manufacturing and Process Scale-Up
Spray drying siRNA-polymeric nanoparticles for dry powder pulmonary delivery.
Wednesday, July 16, 2025
11:27 AM - 11:38 AM EDT
Location: 123
Introduction: RNA-based therapeutics have transformed medicine(1). However, they remain limited to intravenous administration. Inhalation of RNA nanoparticles (NPs) offers advantages such as enhanced organ-specific accumulation. Spray drying technique is a promising strategy to achieve aerodynamic diameters for lung deposition(2). This study focuses on poly(beta aminoester) (pBAE) NPs, which provide benefits like tunable composition and higher drug loading but face stability challenges(3). We optimized spray-drying conditions for siRNA-loaded pBAE NPs and evaluated their potential for pulmonary delivery.
Learning Objectives:
At the completion of this activity, participants will know
Define the proper parameters to successfully spray dry siRNA-pBAE NPs.
Evaluate the aerodynamic requirements of a dry powder to be inhaled.
Demonstrate that spray dried pBAE NPs achieved a gene silencing effect above 90%.
Leonie Deßloch, na – PhD Candidate, Ludwig-Maximilians-Universität München, Germany; Adrian Kromer, na – PhD Candidate, Ludwig-Maximilians-Universität München, Germany; Savador Borrós, Prof. – Director and Prof., Institut Químic de Sarrià (IQS); Olivia Merkel, Prof. – Prof., Ludwig-Maximilians-Universität München, Germany; Cristina Fornaguera, Prof. – Prof., Institut Químic de Sarrià (IQS)
