Thermal Stabilization and Immunogenicity of mRNA Vaccine Delivered by Single-Use Microneedle Patches

Introduction: During a pandemic, specific and effective messenger RNA (mRNA) vaccines have demonstrated therapeutic potential. The demand for multiple injections and the need for cold chain storage have hindered optimal vaccine utilization. The introduction of thermally stable vaccines with a single administration process could revolutionize global immunization. Here, we demonstrate a 3D method for loading mRNA-based vaccines into microneedle array patches with programmed release through efficient liposomal carrier for mRNA vaccines. They are serving as a model for the painless delivery of mRNA vaccines.