Tech Session IV: Alternative Methods to Animal Testing I
mRNA-LNPs Microneedle Delivery in 3D Human Dermal Equivalent Model for Preliminary In-Vitro Testing
Wednesday, July 16, 2025
3:16 PM - 3:27 PM EDT
Location: 125
Introduction: Microneedles (MNs) are a cutting-edge intradermal delivery approach, enabling minimally invasive and efficient skin barrier penetration [1,2]. The proposed MNs encapsulate E-GFP mRNA lipid nanoparticles (LNPs) within the two main compartments of our PLGA microparticle (MP)-based MNs (the fast dissolvable tip and the slow-release MPs). In this study, we advanced MN characterization using an innovative skin-on-a-chip model based on an optically accessible platform integrating the human dermal equivalent (HDE) model[3,4].
Learning Objectives:
At the completion of this activity, participants will know
Evaluate the sustained mRNA delivery by polymeric bicompartmental microneedle
Human dermal equivalent model as suitable in vitro 3D system for drug delivery testing
Evaluate nano-mRNA delivery in microencapsulation strategy in a 3D Human dermal equivalent model.
Roberta Passariello – Technician, Dipartimento di Ingegneria Chimica e dei Materiali, Università degli studi di Napoli Federico II; Daniela Orefice – PhD student, Istituto Italiano di Tecnologia; Atefeh Malek Khatabi – Post Doc, Istituto Italiano di Tecnologia; Alessandro Attanasio – Post Doc, Istituto Italiano di Tecnologia; Stefano Persano – Researcher, Dipartimento di Oncologia ed Emato-Oncologia, Università degli studi di Milano; Giorgia Imparato – Chief Technician, Istituto Italiano di Tecnologia; Raffaele Vecchione – Chief Technician, Istituto Italiano di Tecnologia; Paolo Netti – Professor, Istituto Italiano di Tecnologia
