Tech Session IV: Alternative Methods to Animal Testing I
mRNA-LNPs Microneedle Delivery in 3D Human Dermal Equivalent Model for Preliminary In-Vitro Testing
Wednesday, July 16, 2025
3:16 PM – 3:27 PM EDT
Introduction: Microneedles (MNs) are a cutting-edge intradermal delivery approach, enabling minimally invasive and efficient skin barrier penetration [1,2]. The proposed MNs encapsulate E-GFP mRNA lipid nanoparticles (LNPs) within the two main compartments of our PLGA microparticle (MP)-based MNs (the fast dissolvable tip and the slow-release MPs). In this study, we advanced MN characterization using an innovative skin-on-a-chip model based on an optically accessible platform integrating the human dermal equivalent (HDE) model[3,4].
Learning Objectives:
At the completion of this activity, participants will know
Evaluate the sustained mRNA delivery by polymeric bicompartmental microneedle
Human dermal equivalent model as suitable in vitro 3D system for drug delivery testing
Evaluate nano-mRNA delivery in microencapsulation strategy in a 3D Human dermal equivalent model.
