Tech Session IV: Nanomedicine and Nanoscale Delivery (Focus: Gene)
Dual Centrifugation: A Novel Perspective on LNP Formulation Development
Wednesday, July 16, 2025
3:27 PM – 3:38 PM EDT
Introduction: The administration of RNAs faces challenges such as a poor cellular uptake, rapid degradation and elimination, which limits their clinical application. Lipid nanoparticles (LNPs) proofed to be suitable drug delivery systems (DDS), to address these impediments. [1,2] Our work introduces dual centrifugation (DC) as a novel, rapid, and reproducible technique for formulating small, sterile, and highly concentrated LNPs. This method enables high-throughput screening with up to 40 vials per run and produces homogeneous RNA-loaded nanoparticles within just 7 minutes. [3]
Learning Objectives:
At the completion of this activity, participants will know
... a new formulation technique for the preparation of LNPs
