Advancing RNAi Delivery in Plants through Guanidinylated Polymer Systems

Introduction: RNA interference (RNAi) has shown potential as a sustainable alternative to chemical pesticides to control pests and accommodate the increasing food demand worldwide.(1) However, the efficacy of RNAi is limited due to physical (e.g., cell membrane) and environmental barriers (e.g., sunlight).(2) Polymeric nanocarriers have emerged over the last decade to overcome these barriers. Due to their ability to form polyplexes, they can increase the stability of the nucleic acid and improve its efficacy.(3) The nature of polymeric nanocarriers allows them to be engineered for specific applications.(4)