Rational design of mRNA folding architecture to enhance protein production

Emerging evidence presented that nucleotide sequence variations and lipid nanoparticles can be designed and predicted to generate highly stable and effective mRNA medicines.^1,2 Despite these advances, there remain opportunities to improve mRNA functionality through structural engineering of mRNA molecules. Current studies have demonstrated that folded secondary structure arise from nucleotide pairing, influences RNA stability.^3,4 A completely open question is whether RNA tertiary structures driven by external forces could influence mRNA molecules for expressing proteins.