Hybrid polymer-lipid nanoparticles as innovative transfection vectors for microRNA delivery

Introduction: MicroRNAs (miRNAs) regulate various physiological and pathological processes [1,2]. Thus, several therapeutic strategies based on viral vectors and non-viral transfection agents for miRNAs delivery have been developed. However, viral vectors face limitations due to safety issues and high costs, while commercial lipidic and polymeric transfection agents suffer from poor stability, incomplete loading efficiency, and uncontrolled release kinetics [3]. To address these challenges, novel polymer-lipid nanoparticles (H-NPs) were designed as innovative transfection agent for miRNA delivery.

Letizia Nicoletti – Post doc, Department of Mechanical and Aerospace Engineering (DIMEAS), Politecnico di Torino; Camilla Paoletti – Post doc, Department of Mechanical and Aerospace Engineering (DIMEAS), Politecnico di Torino; Elena Marcello – Researcher, Department of Mechanical and Aerospace Engineering (DIMEAS), Politecnico di Torino; Giovanni Paolo Stola – PhD student, Department of Mechanical and Aerospace Engineering (DIMEAS), Politecnico di Torino; Francesco Schiavone – Post-graduate fellow, Department of Mechanical and Aerospace Engineering (DIMEAS), Politecnico di Torino; Francesca Cossetta – PhD student, Department of Mechanical and Aerospace Engineering (DIMEAS), Politecnico di Torino; Barbara Stella – Professor, University of Turin; Silvia Arpicco – Professor, University of Turin; Clara Mattu – Professor, Department of Mechanical and Aerospace Engineering (DIMEAS), Politecnico di Torino; Valeria Chiono – Professor, Department of Mechanical and Aerospace Engineering (DIMEAS), Politecnico di Torino