Tech Session VI: Nanomedicine and Nanoscale Delivery (Focus: Gene)
High-throughput screening of lipid nanoparticles for lung delivery of mRNA
Thursday, July 17, 2025
6:08 PM – 6:19 PM EDT
Introduction: Lipid nanoparticles (LNPs) have paved the way for nucleic acid delivery. The fine-tuning of LNP composition enables targeted delivery to specific organs and administration via distinct routes1. Intranasal (IN) route provides access to the lungs without systemic exposure. However, determining an effective LNP composition to overcome barriers imposed by this route is a challenge. We utilized DNA barcodes for a high-throughput in vivo screening of a library of LNPs to lungs delivery following IN administration. The top-performing LNPs were further validated in terms of transfection efficiency.
Learning Objectives:
At the completion of this activity, participants will know
DNA barcode screening accelerates the identification of lead LNPs.
Characterizing LNPs in cell culture medium may help in understanding formulation performance.
