Elucidation of Lipid Nanoparticle Fusion Mechanisms Using Artificial Membranes

Introduction: Lipid nanoparticles (LNPs) with target cell tropism are essential components of advanced drug delivery systems (DDS), facilitating the efficient delivery of nucleic acid therapeutics[1]. Optimizing the lipid composition of LNPs offers a promising approach, with the potential to maintain cell tropism while minimizing immunogenicity[2]. In a previous report, we demonstrated that AI-assisted methodologies largely improved the efficiency of lipid composition optimization[3]; however, a deeper understanding of the mechanisms by which lipid composition influences cell tropism is still required.