Tech Session V: Gene Delivery and Gene Editing III
Evaluating the effects of PEGylated lipid shedding on targeted nanomedicines
Thursday, July 17, 2025
10:04 AM – 10:29 AM EDT
Introduction: PEG-lipids are commonly used to conjugate targeting ligands to nanoparticle surfaces to facilitate the targeted delivery of nanomedicines to particular cell types [1]. However, PEG-lipids can desorb during circulation in a phenomenon known as “shedding,” resulting in a loss of targeting capacity [2]. To decouple nanoparticle stability from the effects of shedding, we use highly stable polymersomes self-assembled from polyethylene glycol-block-polypropylene sulfide (PEG-b-PPS) to develop shedding heuristics for chemical properties of PEG-lipids.
Learning Objectives:
At the completion of this activity, participants will know
Determine how FRET can be used to quantify PEG-lipid shedding.
Evaluate how modulating PEG-lipid chemistries affects shedding.
