Liquid Crystalline Nanoparticles as a versatile platform for mRNA delivery
Friday, July 18, 2025
9:35 AM – 9:46 AM EDT
Introduction: Liquid Crystalline Nanoparticles (LCNs) have emerged as a versatile platform for delivering active compounds, including nucleic acids, due to their highly ordered two- and three-dimensional structures (1). Incorporating ionizable lipids, enhances mRNA loading and its endosomal escape, critical for intracellular mRNA delivery (2,3). Here, we developed LCNs with ionizable lipids (L1, SM-102, ALC-0315) to optimize mRNA delivery via intramuscular (IM) administration, evaluating their physicochemical properties and transfection efficiency in vitro and in vivo in animal model.
Learning Objectives:
At the completion of this activity, participants will know
Understand the effect of ionizable lipids on LCNs' properties and mRNA delivery efficiency
Learn LCNs synthesis, characterization, SAXS, and transfection efficiency in vitro and in vivo
Explore LCNs for gene-based vaccines, highlighting the LCN+L1 formulation's improved mRNA delivery
