Tech Session I: Nanomedicine and Nanoscale Delivery (Focus: Gene)
Characterizing CRISPR-Cas ribonucleoprotein delivery with bioreducible polymer nanoparticles
Tuesday, July 15, 2025
10:04 AM - 10:29 AM EDT
Location: 121 B/C
Introduction: Growing adoption of CRISPR-Cas methods has ignited a revolution in genome engineering. The main barrier to further progress is the lagging capability to deliver Cas-derived nucleases and single guide RNAs (sgRNAs) into cells. To address this, we have advanced a bioreducible polymer nanoparticle delivery system that outperforms the gold-standard reagents for delivery of CRISPR-Cas ribonucleoproteins (RNPs) in cells. The nanoparticles efficiently deliver RNPs comprised of Cas9-sgRNA used in classical non-homologous end-joining and homology-directed repair, as well as prime editing RNP payloads.
Learning Objectives:
At the completion of this activity, participants will know
Appreciate a novel approach to effective intracellular delivery of CRISPR-Cas ribonucleoproteins
Understand divergent intracellular trafficking behavior of nanoparticle RNP delivery systems
Recognize the capability of biorducible polymer nanoparticles to mediate more effective gene editing
Miina Ojansivu – Postdoctoral Researcher, Karolinska Institutet; Bernhard Schmierer – Head of Unit, Karolinska Institutet; Molly Stevens – Professor, University of Oxford
