Workshop: Liquid Chromatography to Characterize and Purify your LNP Drug Product

Sponsored By 

Sponsored by: Sartorius BIA Separations

The participants will learn about the advantages of using chromatography to analyze and purify LNP drug products. Specifically, the use cases on monolithic columns will be showcased. A PATfix chromatographic system with an LNP analytical platform will be installed and presented and the use of hardware and software demonstrated. Analytical methods will be shown that determine: RNA quantity and quality within LNP drug products, encapsulation efficiency, size distribution, lipid composition, RNA-lipid adducts and lipid quality. An alternative to existing purification methods for LNPs will be introduced.

Presentations:

• 8:00 AM - 9:20 AM EDT

  Tackling CMC and Manufacturing Challenges of Complex LNP-based Biopharmaceuticals for Oncological, Gene Editing and Vaccine Applications
  

  Speaker: Tristan Kovačič, MSci – Sartorius BIA Separations
• 9:20 AM - 10:40 AM EDT

  Characterization from pDNA to LNP: A Liquid Chromatography Approach for In-Process and Quality Control of LNP-based Biopharmaceuticals
  

  Speaker: Nejc Pavlin, PhD (he/him/his) – Sartorius BIA Separations
• 10:40 AM - 12:00 PM EDT

  End-To-End Manufacturing Process for Improved Recovery and Integrity, Combined With Innovative Analytics of LNP-Based Biopharmaceuticals
  

  Speaker: Mojca Tajnik Sbaizero, PhD – Sartorius BIA Separations