(469) Nanoparticle-stabilized Pickering emulsion for oral vaccine delivery
Introduction: The major challenge in oral vaccine delivery is overcoming gastric acid degradation while ensuring robust immune activation (1). Pickering emulsions, stabilized by solid particles, enhance stability and antigen transport while protecting against gastric degradation (2). Nanoparticles encapsulating the immunostimulant R848 (R848-NP) further improve efficacy by acting as stabilizers and adjuvants (3). Therefore, we designed an R848-NP-stabilized Pickering emulsion (R848-NP@PE-OVA), aiming to enhance antigen stability, uptake, and mucosal immunity.
Learning Objectives:
Demonstrate the formulation and characterization of R848-PLGA-NP@PE-OVA
Describe how R848-PLGA-NP@PE-OVA enhances antigen uptake and dendritic cell activation
Evaluate the in vivo immunostimulatory effects of R848-PLGA-NP@PE-OVA.
