Tech Session I: Nanomedicine and Nanoscale Delivery (Focus: Gene)
Tunable surface charge on polymer nanoparticles and the effect on endothelial cellular drug delivery
Tuesday, July 15, 2025
11:16 AM – 11:27 AM EDT
Introduction: In this study, we investigate how modifications to the cationic family of poly(amine co esters) (PACE) affect the resulting NP surface and what impact that has on cellular uptake for delivering therapeutic nucleic acids (1). We hypothesize that an increase in amine groups will result in enhanced nucleic acid loading, cell uptake, and endosomal escape due to increased ionizable groups and stronger electrostatic interactions (2). We evaluate these measures in endothelial cells, an understudied target of drug delivery.
Learning Objectives:
At the completion of this activity, participants will know
Understand the modification of PACE polymers and further formulation of corresponding PACE NPs.
Demonstrate amino groups density and characterization of surface charge of different PACE NPs.
Evaluate the nucleic acid encapsulation efficiency and cellular uptake of different PACE NPs.
