CRISPR-Cas Delivery Using Lipid-Polymer Nanoplexes in Microneedle Patches for Corneal Dystrophy
Tuesday, July 15, 2025
3:05 PM – 3:16 PM EDT
Introduction: CRISPR-Cas technology has transformed gene editing, presenting significant therapeutic potential for genetic disorders. However, effective delivery of CRISPR-Cas components remains a critical challenge. Nanoplexes formed by novel cationic biodegradable polymer complexing with plasmids and ribonucleoproteins (RNPs) enhances stability and cellular uptake. This approach is relevant for corneal dystrophy, a progressive genetic eye disorder, where targeted gene therapy may yield substantial advancements. Efficient delivery systems are essential for maximizing the efficacy of CRISPR-based therapies.
Learning Objectives:
At the completion of this activity, participants will know
Upon completion, participants will be able to discuss the significance of CRISPR-Cas delivery.
Upon completion, participants will be able to design nanoplex-based drug delivery systems.
Upon completion, participants will be able to assess microneedle patches for ocular gene delivery.
