3D Cell assemblies to evaluate inflammatory response to dual-agent delivery platforms targeting BV

Introduction: Gardnerella is a prevalent vaginal microflora organism in bacterial vaginosis (BV). Coupled with biofilm formation and pH change, Gardnerella evokes an inflammatory response linked to health complications including preterm birth and increased susceptibility to sexually transmitted diseases [1]. Platforms for local delivery of both antibiotic and probiotic have recently been proposed to promote user adherence and avoid recurrence [2]. We designed a novel 3D cell assembly to assess lower FRT cell inflammatory response to Gardnerella and to platforms delivering metronidazole and L. crispatus.