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Nanomedicine and Nanoscale Delivery

Gene Delivery and Gene Editing

Tech Session VI: Nanomedicine and Nanoscale Delivery (Focus: Gene)

mRNA Vaccine Delivery Using Lipid Nanoparticles: Focus on Muscle Cell Involvement

Thursday, July 17, 2025
4:59 PM – 5:24 PM EDT
Introduction:

The success of mRNA-based COVID-19 vaccines has highlighted the therapeutic potential of nucleic acid-based therapies. Lipid nanoparticles (LNPs) have evolved from research tools to clinically validated delivery systems, as seen with Onpattro® and mRNA vaccines. This study aimed to develop mRNA-loaded LNPs to modulate protein expression in skeletal muscle cells, serving as proof-of-concept for vaccine applications. Efficient transfection of muscle cells is crucial for local production of viral antigens and triggering a targeted immune response.

Learning Objectives:

At the completion of this activity, participants will know
  • Learn how LNPs efficiently deliver mRNA in muscle cells in vitro, ex vivo, and in vivo.
  • Explore LNPs’ potential in modulating protein expression for targeted vaccine applications.
  • Evaluate mRNA-LNPs’ effectiveness in eliciting immune responses through antigen expression.