Unraveling endosomal escape: mechanistic insights into lipid nanoparticle-mediated RNA delivery
Tuesday, July 15, 2025
3:49 PM - 4:00 PM EDT
Location: 119 A
Sponsored By
Introduction: Lipid nanoparticles (LNPs) represent the most promising platform for RNA delivery, yet their therapeutic efficacy is often limited by inefficient endosomal escape following cellular uptake [1-3]. Understanding the mechanisms that drive endosomal escape is critical to overcoming this barrier and optimizing LNP performance [4, 5]. Current models struggle to isolate and deconstruct the molecular events driving RNA release, leaving a critical gap in our understanding of this process.
Learning Objectives:
At the completion of this activity, participants will know
1. Decode the molecular mechanisms underpinning LNP-mediated RNA delivery using GUV systems
2. Identify design parameters and environmental conditions that optimize RNA release.
3. Translate insights from GUV models to cellular systems to enhance therapeutic development
Emeline Bonsergent, NA – Postdoc, MPI-CBG; Lisa Redlingshöfer, NA – Postdoc, MPI-CBG; Anupam Singh, NA – Postdoc, MPI-CBG; Marino Zerial, NA – Group Leader and Director, MPI-CBG
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