Introduction: Murine hepatitis virus 3 (MHV-3), a model for severe viral infection, is transmitted via the respiratory route and triggers a "cytokine storm"1, causing immune cell infiltration into the liver, vascular damage, and worsened outcomes2. Targeting E-selectin, which mediates leukocyte recruitment to inflamed tissues3, offers a strategy to mitigate liver inflammation, and hepatocytes death. Small interfering RNA (siRNA) targeting E-selectin can precisely silence its expression and nanoparticles (NPs) offer a promising solution by protecting siRNA, enabling targeted delivery to endothelial cells4.
Learning Objectives:
Understand the role of e-selectin in inflammation and liver damage of MHV-3-induced cytokine storm.
Explore the synthesis and characterization of hybrid NP-based systems for targeted siRNA delivery
Evaluate the effects of e-selectin siRNA administration on inflammatory cytokines levels.
Lays Guimarães – PhD student, UFMG; Pedro Augusto Costa – Post-doc, UFMG; Filipe de Souza – PhD student, UFMG; Natália Silva – PhD student, UFMG; Maria Marta Figueiredo – Professor, UEMG; Sérgio Ricardo Scalzo – Post-doc, UFMG; Walison da Silva – PhD student, UFMG; Ana Luiza Castro – PhD student, UFMG; Mauro Teixeira – Professor, UFMG; Vivian Costa – Professor, UFMG; Pedro Guimarães – Professor, UFMG
