Introduction: Murine hepatitis virus 3 (MHV-3), a model for severe viral infection, is transmitted via the respiratory route and triggers a "cytokine storm"1, causing immune cell infiltration into the liver, vascular damage, and worsened outcomes2. Targeting E-selectin, which mediates leukocyte recruitment to inflamed tissues3, offers a strategy to mitigate liver inflammation, and hepatocytes death. Small interfering RNA (siRNA) targeting E-selectin can precisely silence its expression and nanoparticles (NPs) offer a promising solution by protecting siRNA, enabling targeted delivery to endothelial cells4.
Learning Objectives:
Understand the role of e-selectin in inflammation and liver damage of MHV-3-induced cytokine storm.
Explore the synthesis and characterization of hybrid NP-based systems for targeted siRNA delivery
Evaluate the effects of e-selectin siRNA administration on inflammatory cytokines levels.
