(255) Biocalorimetry to predict bacterial extracellular vesicle production to improve biomanufacturing

Introduction: Bacterial extracellular vesicles (bEVs) are cell derived nanoparticles involved in microbe-host communication. Previously, we demonstrated compatibility, uptake, and mobility of Lactobacillus spp. derived bEVs in the host environment, presenting bEVs as a promising therapeutic modality. However, manufacturing bEVs to support translation of bEV-based therapeutics is a challenge. Here, we investigate effects of external factors on bEV biogenesis to inform manufacturing. Further, we use biocalorimetry to predict optimal bEV harvest time. Our work has important implications for bEV manufacturing.