(358) Marginated Neutrophils Compete for Pulmonary Endothelial-targeted Nanoparticles, part of the RES

Introduction: LNPs are promising for delivering small-molecule drugs and RNA to target organs. Conjugation of nanoparticles with antibodies against cell adhesion molecules localizes LNPs to the lungs. These LNPs have been assumed to target pulmonary endothelial cells. However, “endothelial-targeted LNPs” also target the lung’s marginated neutrophils. This phenomenon is generalized across diverse nanomaterials and targeting moieties. This is seen in ex vivo human lungs and in vivo healthy mice, with an increase in marginated neutrophil uptake of nanoparticles caused by local or distant inflammation