High-resolution screening of barcoded lipid nanoparticles in tissue cleared mice

Introduction: Current LNP screenings lack multiplexing capabilities, only allowing for a small number of formulations to be tested in a single animal. This restriction increases costs, reduces throughput and requires the use of a large number of animals. Advances in molecular barcoding enabled the evaluation of multiple formulations in a single mouse(1). However, they still lack spatial information and rely on low-resolution imaging for selecting the region of interest(ROI). Advanced tools are needed to combine molecular and spatial information to provide detailed insights into biodistribution.